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ELISA-ssDNA monoclonal antibody detection of apoptosis induced by CBD on glioma cells. Cells were untreated (C) or exposed to CBD where can i buy cannabidiol oil in north carolina (10 ?M and 25 ?M) for 24 h as previously described (see Materials and Methods). Cells treated with hyperthermia (HT; 56C for 1 h followed by an incubation at 37C for 1 h) were used as necrotic cells-negative control of apoptosis.