A similar effect has been reported by another research group in mice after middle cerebral artery occlusion; the neuroprotective action of CBD being unaffected by CB1 receptor is cbd legal in indiana review blockade.92 The same legal cbd legal in illinois research group has verified that this effect was inhibited by WAY100135 a serotonin Legal Cannabinoid Anandamide 5-hydroxytriptamine 1A (5-HT1A) receptor antagonist but not by capsazepine a vanilloid receptor antagonist suggesting that the neuroprotective effect of CBD may be due to the increase in cerebral blood flow mediated by the serotonergic 5-HT1A receptor.93 Experimental evidence has suggested that beyond this order hemp oil sale usa action on the 5-HT1A receptor the protective effect of CBD on ischemic injury is also secondary to its anti-inflammatory action.94 In another study the same research group reported that while repeated treatment with delta9-THC leads to the development of tolerance for this neuroprotective effect this phenomenon is not observed with CBD.95 CBD has been studied for ischemic heart diseases in rats.96 The left anterior descending coronary artery was transiently obstructed for 30 min and the rats were treated for 7 days with CBD (5 mg/kg ip) or vehicle. Legal Cannabinoid Anandamide cardiac function was studied by echocardiography and showed preservation of shortening fraction in CBD-treated animals. Infarct size was reduced by 66% in CBD-treated animals and this effect was associated with reduction of myocardial inflammation and reduction of IL-6 levels. In isolated hearts no significant difference was detected between rats that received CBD or vehicle regarding: infarct size left ventricular developed pressures during ischemia and reperfusion or coronary flow. This study shows that CBD induces a substantial cardioprotective effect but only in vivo.
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