We found that between 12 and 23 days of treatment CBD-treated mice had significantly smaller tumors than did control mice ( Fig. High Cannabidiol Low Thc Strains Colorado Vape 7 ). The regression was of about 70% at day 18 (572 147 mm3 n = 7 in CBD-treated mice versus 1765 259 mm3 n = 7 in control mice) although at 23 days of treatment this effect appeared weaker with an inhibition of the tumor growth of about 50% as compared with control animals (1210 210 mm3 n = 7 in CBD-treated mice versus 2212 256 mm3 n = 7 in control mice). Fig. 7. CBD effect on subcutaneous U87 glioma cell growth. CBD (0.
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The cell pellet was gently resuspended in 1 ml of hypotonic fluorochrome solution propidium iodide (PI) 50 ?g/ml in 0.1% sodium citrate plus 0.1% Triton X-100; Sigma-Aldrich. Cells were analyzed after a minimum of 30 min rsho high cbd hemp oils cbd of incubation in the dark at room temperature and apoptosis was detected in individual cells using a flow cytometer (equipped with a single 488-nm argon laser; BD Biosciences San Jose CA) by reduced fluorescence of PI in apoptotic nuclei. Briefly following the manufacturer’s instructions cells were seeded at 8000 cells/well in a 96-multiwell plate. After the incubation with the drug the plates were centrifuged at 200g for 5 min and the cells were fixed with 80% methanol in PBS for 30 min at room temperature.
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