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CBD was directly loaded onto the liquid pad and vaporised at two different temperatures 210C and 230C into three subsequent balloons (to capture as much CBD vapour as possible) Pilot study 1; see Additional file 1 Next higher doses of crystalline CBD – 200mg and 300mg – were attempted to be vaporised into a single standard balloon at 230C Pilot study 2; see Additional file 1 In Pilot study 3 see Additional file 1 200mg of crystalline CBD was vaporised at 230C into standard size and extra large (XL; 90cm) size balloons to determine whether the amount delivered could be increased by allowing a longer time for cbd legal in virginia compounds to vaporise. Finally we proceeded to establish whether CBD in ethanolic solution of similar concentration would vaporise similarly to what we had established for crystalline CBD Pilot study 4; see Additional file 1 This was deemed to be particularly pertinent since pure THC is generally vaporised dissolved in ethanol and it would be desirable for randomised administration studies of both compounds to humans to deliver each compound similarly and enable ethanol to be used in a placebo condition. Due to the resinous sticky nature of CBD we started with 100mg CBD dissolved in a 10% ethanolic solution loaded onto the liquid pad of the vaporiser by cannabidiol protects liver 2017 pipetting 1ml of solution in several separate aliquots at a time in order to prevent overloading of the liquid pad and vaporising ethanol each time at 100C for 30sec. A further aim for Pilot study 4 was to assess the potential for co-administration of THC and CBD through the vaporiser. 10mg THC (0.4ml from a 4% ethanolic solution) was vaporised alone and in combination with the 100mg CBD
into a normal size balloon at 230C. This series of preliminary studies was used to determine the optimum dose delivery and vaporisation protocols for planned use in a randomised controlled trial (RCT) of cannabinoid (THC and CBD) administration to humans 18 Final confirmatory and replication studies were undertaken using doses described in Table 1 as 6 separate experiments repeated in triplicate with results described below. Confirmatory/replication studies Having identified a range of properties pertinent to optimising vaporisation of CBD we then selected specific doses for confirmation and replication toward the planned RCT.
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